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  • Essay / Microbiology: microorganisms that coexist with life...

    A diverse collection of microorganisms coexist with life on Earth; most of them are plant or ocean dwellers, many are the normal flora of humans, and some live in remote locations in the most extreme habitats. Although they are ubiquitous in nature and generally harmless, bacteria receive special attention because they cause disease in humans. Correct identification of a microorganism allows proper investigation of a particular species and prevention or treatment of disease if necessary. During the laboratory, students were asked to choose a test tube inoculated with an unknown organism and then run a series of appropriate laboratory tests to correctly identify the organism. RESULTS Unknown 3 was received in Trypticase Soy Broth. A Gram test was immediately performed to reveal that the bacteria was a Gram-positive cocci. Once morphology and Gram staining were determined and the presence of growth on a Trypticase Soy Agar slant was apparent, two tests were used to refine the list of possible organisms; these tests were the methyl red test and urea hydrolysis. The methyl red test is used to distinguish the ability of an organism to ferment a mixed acid, which is verified by the color change of the methyl red broth after the addition of a reagent. There was no change in color of the broth, indicating a negative result. Unknown 3 was also inoculated into urease broth, which tested for the presence of urease, an enzyme responsible for the hydrolysis of urea to ammonia and carbon dioxide. If the urea had metabolized, indicating a positive test, the broth would have turned pink. The test was negative. The figures below provide a summary and complete the analysis; Figure 1 describes the purpose of the test, the results and its implications......the middle of the article......s results in the identification of the organism Micrococcus roseus.REFERENCES1. LeBeoffe, Michael J. (2012). Brief theory and application of microbiology laboratory. 2nd edition. Englewood, CO: Morton Publishing Company.2. Nester, Eugene. (2012). The diversity of prokaryotic organisms. Microbiology: a human perspective. 7th edition (pages 264, 275). New York, New York: McGraw-Hill.3. Todar, Kenneth. (2012). Staphylococcus. Retrieved from http://textbookofbacteriology.net/staph.html4. Tortora, GĂ©rard, B. Funke and C. Case. (2010). Interaction between the microbe and the host: normal microbiota. Microbiology: an introduction. 10th edition. (pages 401 to 403). San Francisco, CA: Pearson Education, Inc.5. Wistreich, Georges. (2007). Microbiological Perspectives: A Photographic Study of the Microbial World. 2nd ed. Upper Saddle River, NJ: Pearson Education, Inc..