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  • Essay / HIV testing: analysis of the relationship between laboratory antibody and antigen reactions

    Table of contentsMaterials and methodsResultsDiscussionConclusionThis report discusses the relationship between laboratory antibody and antigen reactions. HIV tests were carried out at Chinhoyi Provincial Hospital with the help of laboratory assistants. The tests were carried out using rapid test kits which require a short period of just fifteen minutes to produce results. IntroductionIt is well established that approximately 20-80% of people infected with HIV in different parts of the world are unaware of their HIV status. It is therefore important to seize every opportunity to offer testing to people who are unaware of their status. Laboratories can play an important role in managing testing procedures by having clear and detailed test ordering protocols (e.g., providing relevant clinical information on forms) and carefully evaluating all screening test results of HIV according to the most recent diagnostic protocols. Say no. to plagiarism. Get a tailor-made essay on “Why violent video games should not be banned”? Get the original essay Materials and methods HIV rapid test kits Alcohol Cotton/wool gauze Sterile lancets Sharps bin or lancet disinfectant pot Pen for marking or labelingGlovesAprons or lab coatsTimer, clock, or wristwatch with minute handPaper towels (for bench covering, cleaning, and hand washing)Soap for hand washingWaterproof bag for containing or moving hazardous waste biological labeled for incinerationDisinfectant e.g. Jik, dressings or chlorox dressingsPositive and negative controlsStandard operating procedures. Results A negative control produced a single faint line on the control section of the test kit, indicating that the sample was negative for HIV. A positive control sample produces faint double lines on the control section of the test kit and on the positive section of the test kit. This indicated that the blood sample was HIV positive. The unknown sample only showed a single faint line on the test kit's control unit. So an HIV negative result. DiscussionWhen the test sample is negative for HIV, only a single faint line is noted on the test kit portion, but if the test sample is found to be positive, double lines should be seen on both the control part and on the positive part of the rapid test. test kit. Note that any result indicating samples are positive or negative without a line observed on the test kit control unit is rendered null, why in bold. Indeed, there is no evidence to rule out cases such as mechanical defects, manufacturer defects, and even expiration date being important to kit performance. It is therefore very important to ensure that all test kits are working properly before confirming samples. be positive or negative. Conclusion To ensure certainty of results, it is advisable to perform a series of the same experiment to reduce the effects of operational errors on the results. The use of known control samples, known as negative and positive, will confirm the effectiveness of the test kits and also increase the operator's confidence in the veracity of the results obtained. Additionally, the use of SOPs would minimize the risk of procedural errors..