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Essay / 010).Mycolic acids can be recognized based on their chain length (C70 - C90), the presence of double bonds, their long side chain, their additional oxygen or methyl functions. The three main mycobacterial mycolate classes can be separated by “normal phase” HPLC, but the characteristic peaks of MTBC cannot be identified. However, “reserve phase” HPLC (rpHPLC), can separate mycolic acids based on their chain length and paper length......, D. (1999) Genotypic analysis of Mycobacterium tuberculosis from medieval human remains, Microbiology 145: 899-904;22. Scorpio, A., Zhang, Y. (1996) Mutations in pncA, a gene encoding pyrazinamidase/nicotinamidase, cause resistance to the antituberculosis drug pyrazinamide in tubercle bacilli, Nat Med 2(6): 662-667;23 . Zimhony, O., Cox, JS, Welch, JT, Vilcheze, C. and Jacobs, WR, Jr. (2000) Pyrazinamide inhibits eukaryotic fatty acid synthetase I (FASI) of Mycobacterium tuberculosis, Nat Med 6: 1043 –1047;24. Hofreiter, M., Serre, D., Poinar, H.N., Kuch, M., Pääbo, S. (2001) Ancient DNA, Nat Rev 2(353): 353-359;25. Shi, W., Zhang, X., Jiang, X., Ruan, H. et al. (2011) Pyrazinamide inhibits trans-translation in Mycobacterium tuberculosis: a potential mechanism to shorten the duration of anti-tuberculosis chemotherapy, Science 333(6049): 1630–1632.
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