Title: Research Question: Can modifying the genetic material of bacteria create new characteristics through gene transfer?Introduction: Prokaryotic cells have no nucleus or inner membrane. For this reason, they can be classified as bacteria. Prokaryotic cells are similar in structure to eukaryotic cells, but the DNA floats in a nucleoid. The transformed bacteria consist of a recombinant plasmid and one or more host cells. If the bacteria's plasmid has a useful gene, it can be very beneficial to the bacteria. Scientists can combine the new DNA they want to replicate with the plasmid, making it available in large quantities. The bacteria can pass the plasmid to another bacteria, creating many lawns or colonies, which is called bacterial transformation. A plasmid is a small circular piece of double-stranded DNA. Plasmid DNA contains a gene that is shown by the bacteria. Scientists can modify plasmids and create new genes. This shows that bacteria can acquire new information and genetic characteristics. In bacteria with a plasmid in transformed bacterial cells, ampicillin and other antibiotics cannot destroy their cell wall, usually by using the beta-lactam chemical ring. The ampicillin resistance gene in a plasmid encodes a protein called beta-lactamase, which is an enzyme that destroys the activity of ampicillin by breaking down the beta-lactam ring. These transformed bacterial cells can resist the effects of beta-lactam antibiotics. Plasmids are Even though the DNA is free-floating, if a gene is important, the DNA can be encoded. Independent variable: The plasmid Dependent variable: The type of growth of the bacteria in each dish Controlled variables: The amount of plasmid added to +.. . ... middle of paper ......mpicillin, x-gal and +plasmid. Figure 3 shows columns of yellow bacterial growth when LB and +plasmid are present. Figure 4 shows lawns of yellow bacterial growth when LB and –plasmid are present. Figure 5 shows no signs of bacterial growth with x-gal, ampicillin, LB, and –plasmid. Figure 6 shows a small lawn on yellow bacterial growth with ampicillin, LB and –plasmid. colony Next to the blue colonies there are smaller white colonies. These bacteria did not transform, but survived. In the area around the blue colony there is no ampicillin. They find themselves in an area without an amplifier. and starts to grow. They develop after the blue cell has killed the antibiotic. They are called satellite colonies.