The calla lily (Zantedeschia spp.), a genus of the Araceae family, is a perennial flowering plant and grows naturally in Central and South Africa (Wei et al., 2017). This genre consists of two sections. The first group is Zantedeschia aethiopica, known as the white calla lily, which has a rhizomatous storage organ and is evergreen. The second group is colorful calla lilies and they have a tuberous storage organ and require a dormant period (Zhang et al., 2011). Calla lilies are commercially available as cut flowers and potted plants. These flowers have a high commercial value in the market due to their attractive inflorescence (Jonytiene et al., 2017). According to 2014 statistics, the turnover of calla lily was 19 million euros (euro) in the Netherlands (Floraholland facts and figures, 2014). It contributed to a substantial portion of horticultural export earnings in the Netherlands and America. The yield of colorful calla lily tubers in these two countries has increased significantly in recent years to meet demand from overseas markets, especially in Asia (Wei et al., 2017). In most cases, conventional propagation of calla lily involves dividing the tuber, but this method requires high skills and also provokes the development of soft root rot. Bacterial soft rot caused by Erwinia carotovora subsp.Say no to plagiarism. Get a tailor-made essay on “Why Violent Video Games Should Not Be Banned”? Get the original essayCarotovora is a major problem in Zantedeschia spp. Infection occurs at all stages of the life cycle, from the growing period of plants in the field to tubers in the store, and it spreads rapidly in calla lilies (Wright et al., 2002). The final stage is the destruction of the plant and death; losses can approach 100% in the event of a severe epidemic. For a long time, this disease has been recognized as a major cause and fatal disease for which the calla industry has not made progress (Cho et al., 2013). It should be noted that the acquisition of vigorous plants is considered essential for satisfactory flower production. Knowledge and application of principles and techniques from the choice of propagation material to plant management are necessary to produce high quality plants (Oliveira, 2007). Therefore, growers are looking for a way to reduce problems and produce high-quality calla lily plants. The use of in vitro culture methods can be effective in reducing losses and increasing quality (Cheng et al., 2003). Tissue culture propagation is the most suitable method to quickly obtain numerous pathogen-free plants (Kulpa, 2016). In fact, micropropagation is widely used for commercial purposes as a method of vegetative propagation from different explants. The successful use of tissue culture techniques has been heralded in several reports for the rapid spread of the genus Zantedeschia. Chang et al (2003) presented a method for micropropagation of Zantedeschia albomaculata using shoot tip proliferation. The results showed that 6-benzyladenine (BA) and thidiazuron (TDZ) were more effective and the optimal concentrations of BA or TDZ were 8.87 mM and 4.54 mM, respectively. Koech et al (2005) described a protocol for in vitro shoot regeneration of Zantedeschia albomaculata, using explants of shoot primordia. Shoot explants can be used to produce.