Visualization of the water conduction system of woody plants is an essential parameter for estimating water alteration (Wullschleger et al. 2001, Kumagai et al. 2005) and the anatomical changes caused by implications promoting seedling growth. in reforestation ecosystems. The xylem, which is the main part of the water conduction system, as proven by experimental evidence in plants, regulates water transport regulated by the differentiation of water potential between the air surrounding the leaves and the soil surrounding the roots (Myburg and Sederoff, 2001). Say no to plagiarism. Get a tailor-made essay on “Why Violent Video Games Should Not Be Banned”? Get the original essay Several studies have been conducted to visualize water pathways in xylem by different methods, including dye injection (Umebayashi et al. 2007; Maton and Gartner 2005). , injection of radioactive dye (Postlethwait and Rogers 1958), and observation of water in plants by NMR imaging (Utsuzawa et al. 2005). However, these methods are difficult and expensive to implement on small plants. Additionally, different dye solutions were used to visualize the water-conducting system (Jupa et al. 2015). Acid fuchsion (Maton and Gartner, 2005) and safranin o (Schulte and Brooks, 2003) dyes were used to visualize the water-conducting system in pine seedlings. However, the protocols belonging to these dyes were not clearly detailed and usable to have a clear visual. observation to measure the diameter of hydraulically active xylem. Diameter and percentage of hydraulically active xylem can be one of the best indicators, which can be anatomically improved by implementations in the nursery growth phase, show and determine seedling survival and growth performance in difficult planting sites. We have little understanding of how environmental stresses such as drought affect xylem hydraulics in conifers; especially in Pinus ponderosa. Because water-conducting fabrics and hydraulics are key to understanding how producing seedlings suitable for planting sites is important to improving post-planting success. Ponderosa pine seedling morphology, such as improved seedling height and collar diameter, was favored by initial nursery practices. The xylem diameter and hydraulic activity of the total xylem quantity should be varied with initial nursery practices. Keep in mind: this is just a sample. Get a personalized article from our expert writers now. Get a Custom Essay So, to test this prediction, crystal violet Staning was used to visualize the hydraulic activity and diameter of ponderosa pine seedlings in Chapter 1, which resulted in a failure to visualize xylem diameter. The objective of this study is to develop the best combination of dye staining using acid fuchsin and safranin o in three concentrations (0.01%, 0.1% and 0.5%), three periods of dye exposure (10 min, 20 min, and 30 min), and two additional time series for maintaining seedlings in distilled water (0 min and 5 min). These dye-colored combinations, including time series, were compared using light microscope images based on a clarity-ranking procedure of the hydraulically active xylem of ponderosa pine seedlings...