Grape seed extract (GSE) is a complex mixture of several compounds, mainly represented by polyphenols and phenolic acids. The aim of the present study was to determine the safe dose of GSE that can be used in a mouse model and to evaluate the potential toxicity that can be triggered by different concentrations of this substance. Forty-two mice were divided into 7 groups; groups 1 served as a control, while animals in groups 2, 3, 4 were treated respectively with 200, 400 and 800 mg/kg of black grape seeds, and the remaining groups, including 5, 6 and 7, were treated with 200, 400 and 800 mg/kg bw of red grape seeds respectively. Say no to plagiarism. Get a tailor-made essay on “Why Violent Video Games Should Not Be Banned”? Get the original essay. Animals were examined daily for any telltale signs of activity alterations and toxicity, as well as body weight throughout the experiment for 21 days. At the end of the study, the mice were euthanized and the liver, kidneys, spleen, lungs, and brain were collected for histopathological examination. The results obtained in this study revealed that GSE doses of 200 and 400 mg/kg body weight were safe compared to the dose of 800 mg/kg body weight, which resulted in reduced body weight gain. and histopathological changes. No deaths occurred in any group. Keywords: Albino mouse, Black grape seed, Red grape seed; Sharbasher village. Introduction: Plants are capable of producing a large number of chemical compounds with important biological effects and have been used to make a wide variety of drugs since the creation of man. Grapes, the berries of Vitis vinifera, are considered conventional fruits enjoyed around the world. world. The main composition of grape marc is skin, steam and seeds [4]. The chemical composition of fresh grapes is approximately 70-80% water and dissolved solids such as sugars, phenolic compounds, nitrogen compounds, organic acids, minerals, aromatic compounds, pectic substances. The unique combination of phytochemicals found in grapes includes a variety of bioactive compounds such as simple phenolics, flavonoids, anthocyanins, stilbenes, proanthocyanidins, and vitamin E. Grape seeds are considered a waste product in industry and are made up of proteins, carbohydrates, lipids and 5-8% polyphenols depending on the type of grape. The composition of grape seeds differs considerably between different cultivars. Additionally, multiple environmental and biological factors, such as light, drought, high salinity, cold, metal ions, pollutants, xenobiotics, toxins, experimental manipulations, pathogen infections and plant aging can affect yields. and seed quality. A multitude of flavonoids are contained in GSE. The most abundant of these are proanthocyanidins, which are oligomers of monomeric flavan-3-ol units linked by carbon-carbon bonds. Keep in mind: this is just a sample. Get a personalized article from our expert writers now. Get a Personalized Essay Flavonoids are the most abundant biologically active phytonutrients among the polyphenols present in grapes, possessing cardioprotective, neuroprotective, antimicrobial, anti-aging, cardioprotective, antioxidant, anti-inflammatory,..